منابع مشابه
Peroxisomes and Disease - An Overview
Peroxisomes are indispensable for human health and development. They represent ubiquitous subcellular organelles which compartmentalize enzymes responsible for several crucial metabolic processes such as β-oxidation of specific fatty acids, biosynthesis of ether phospholipids and metabolism of reactive oxygen species. Peroxisomes are highly flexible organelles that rapidly assemble, multiply an...
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Immunofluorescence and a new histochemical technique were employed to assay 226 breast cancer specimens for estrogen receptor. Results showed an overall correlation of 91 percent when compared to those of biochemical assays. The histochemical technique is rapid, easy to perform and reveals the same parameters as does immunofluorescence without the need for antiserum. Tumor cell receptor heterog...
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Seeing peroxisomes t about the same time that Christian de Duve and his colleagues were describing the biochemistry of lysosomes (see “Catching sight of lysosomes” JCB 168: 174), they biochemically identified (Baudhuin et al., 1965) and purified (Leighton et al., 1968) another enzyme-containing organelle. Initially the organelle was known as the microbody, and de Duve declined to give it a more...
متن کاملPartial disassembly of peroxisomes
Rat liver peroxisomes were subjected to a variety of procedures intended to partially disassemble or damage them; the effects were analyzed by recentrifugation into sucrose gradients, enzyme analyses, electron microscopy, and SDS PAGE. Freezing and thawing or mild sonication released some matrix proteins and produced apparently intact peroxisomal "ghosts" with crystalloid cores and some fuzzy f...
متن کاملHistochemistry of bone marrow aspirations.
The accuracy of diagnosis of hematological diseases from bone marrow aspirates and peripheral blood smears is considerably improved by use of histochemical stains. As hematopoietic cells differentiate and mature from the pluripotent stem cell and are committed to a particular cell line, the committed cell lines produce different substances, particularly enzymes, which can be identified by vario...
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ژورنال
عنوان ژورنال: ACTA HISTOCHEMICA ET CYTOCHEMICA
سال: 1995
ISSN: 0044-5991,1347-5800
DOI: 10.1267/ahc.28.165